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Additionally, mesenchymal markers like Vimentin and α-SMA were also diminished, while epithelial markers such as E-cadherin, Occludin, and ZO-1 were elevated, suggesting a disrupted EMT process.

Zonula occludens-1 (ZO-1) localization was observed by immunofluorescence staining. Expression of phosphor-p65 (p-p65), p65, vascular cell adhesion molecule-1 (VCAM-1), and ZO-1 in the kidney were ...

A full list of antibodies, clones and fluorophores can be found in online supplementary table 1. For immunofluorescence microscopy, frozen sections of EST were incubated with an Alexa Fluor 647 ...

1.0±0.2 fold; p=0.03). As the phosphorylation status of OCLN has been linked to its distribution in epithelial cells,28 we assessed OCLN staining by confocal immunofluorescence. IBS-D samples ...

For immunofluorescence analysis, HEK293T cells were seeded into 6-well plates to 90% confluence in 24 hours. Transfections were performed using 1 µg of vector (p513-KCNA1 or pKCNA1-EGFP, wild-type or ...