she submerges the rat's tail in a cup of warm water (50°C) for 3–5 min. Next, she rubs gently both lateral sides of the tail unidirectionally (downward) with a piece of warm, wet paper towel ...

Techniques for blood collection from the rat include puncture of the heart, retro-orbital plexus, jugular vein, saphenous vein, tail blood vessels, carotid artery, abdominal aorta, and vena cava.