If you see bubbles in the syringe, empty the syringe into a waste beaker and redo Step #4. The sample will be injected to a port at the top of the HPLC. 6. Check that the knob, on the port at the top ...

Store the fractions at 4 °C for subsequent RP-HPLC or SDS-PAGE or at −20 °C ... Clarification by filtration through 0.2-μm syringe filter units can also be performed; however, some sample ...